Coding
Part:BBa_K3139012:Design
Designed by: Jiapei Han Group: iGEM19_NAU-CHINA (2019-10-03)
TEVpS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 322
Illegal SapI.rc site found at 670
Design Notes
We need to control the ratio of enzyme to fusion protein expression,to make the best effect.
Source
We found the coding sequence of TEVpS on NCBI,then send it for syhthesis.
References
[1]Cesaratto F , Burrone O R , Petris G . Tobacco Etch Virus protease: A shortcut across biotechnologies[J]. Journal of Biotechnology, 2016, 231.
[2]Jianguo Y , Xiaqing X , Nan X , et al. Polyprotein strategy for stoichiometric assembly of nitrogen fixation components for synthetic biology[J]. Proceedings of the National Academy of Sciences, 2018:201804992-.